CYTOTOXIC EFFECT OF GINGER AND BLUE PEA HERBAL FORMULATION BASED MOUTHWASH - AN IN VITRO STUDY.

INTRODUCTION: Herbal mouthwash made from natural sources is used in periodontal therapy controlling bleeding, inflammation. The efficacy of herbal mouthwash is effective in reducing plaque and gingivitis when compared to chlorhexidine mouthwash. Clitoria ternatea (blue pea) to be an effective natural remedy for a variety of ailments. Aim of the study is to evaluate the cytotoxic effect of ginger and blue pea based herbal mouth wash. MATERIALS AND METHODS: 100ml of distilled water was taken in a measuring cylinder and 1g of neem and stevia was measured and taken using a weighing machine. The temperature of the heating mantle was set to about 50 degrees celsius and the time taken to heat is about 5 - 10 minutes.A six-well ELISA plate was taken and 5 mL of artificial seawater was added to each of the 6 wells and 10 nauplii are added to each well. Then, 5 different concentrations of dried blue pea and ginger were introduced to each of the 5 wells. RESULTS: At incubation time after 24 hours, 100 % of live nauplii were present in 5ul concentration. And 90% of live nauplii were present in 10ul concentration. At incubation time after 48 hours, 100% of live nauplii were present in 5ul concentration and 90 % of live nauplii were present in 10 ul concentration. And 80 % of live nauplii were present in 20 ul, 40 ul, 80ul concentration. CONCLUSION: In this study ginger and blue pea herbal formulation showed good cytotoxic effect as the concentration of the formulation increased, number of nauplis decreased. [ABSTRACT FROM AUTHOR]